Article
Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species.
FEMS microbiology letters - 1 Jun 2006
Payne George W, Ramette Alban, Rose Helen L, Weightman Andrew J, Jones T Hefin, Tiedje James M, Mahenthiralingam Eshwar
Abstract excerpt
Burkholderia species are widely distributed in the natural environment. We evaluated the use of the recA gene in a cultivation-independent approach to examine the Burkholderia diversity associated with the maize rhizosphere. Two types of recA gene library were constructed, one with broad-specificity recA primers (BUR1 and BUR2) and a second from the products of nested PCRs using Burkholderia-specific primers...
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