Article
Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine.
Veterinary microbiology - 15 Jun 2006
Ferrão-Beck Lénia, Cardoso Regina, Muñoz Pilar María, de Miguel María Jesús, Albert David, Ferreira Ana Cristina, Marín Clara María, Thiébaud Martine, Jacques Isabelle, Grayon Maggy, Zygmunt Michel S, Garin-Bastuji Bruno, Blasco Jose María, Sá Maria Inácia
Abstract excerpt
Swine brucellosis is caused by the biovars 1, 2 and 3 of Brucella suis the identification of which up to now relies on microbiological tests lacking adequate specificity together with time consuming and expensive molecular procedures. Based on sequence variation of the omp2b gene, we have developed a four primer set multiplex PCR assay that was tested for polymorphism analysis of B. suis biovars causing...
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