Article
Chromophore attachment in phycocyanin. Functional amino acids of phycocyanobilin--alpha-phycocyanin lyase and evidence for chromophore binding.
The FEBS journal - 1 Mar 2006
Zhao Kai-Hong, Wu Dong, Zhang Ling, Zhou Ming, Böhm Stephan, Bubenzer Claudia, Scheer Hugo
Abstract excerpt
Covalent attachment of phycocyanobilin (PCB) to the alpha-subunit of C-phycocyanin, CpcA, is catalysed by the heterodimeric PCB : CpcA lyase, CpcE/F [Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA & Glazer AN (1992) Proc Natl Acad Sci USA89, 7017-7021]. CpcE and CpcF of the cyanobacterium, Mastigocladus laminosus PCC 7603, form a 1 : 1 complex. Lyase-mutants were constructed to probe functional domains. When...
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