Article
Latent nitrate transport activity of a novel sulfate permease-like protein of the cyanobacterium Synechococcus elongatus.
The Journal of biological chemistry - 3 Mar 2006
Maeda Shin-ichi, Sugita Chieko, Sugita Mamoru, Omata Tatsuo
Abstract excerpt
The Synechococcus elongatus mutant lacking the nrtABCD gene cluster (NA3) is defective in active nitrate transport and requires high nitrate concentrations (>30 mm) for sustained growth. Prolonged incubation of NA3 in medium containing 2 mm nitrate led to isolation of a pseudorevertant (NA3R) capable of transport of millimolar concentrations of nitrate, from which three mutants with improved affinity for nitrate...
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