Article
Use of PCR amplification of tRNA gene-linked short tandem repeats for genotyping Entamoeba histolytica.
Journal of clinical microbiology - 1 Dec 2005
Ali Ibne Karim M, Zaki Mehreen, Clark C Graham
Abstract excerpt
We have developed a reliable method for PCR-based genotyping of Entamoeba histolytica based on variation in the numbers of short tandem repeats that are linked to tRNA genes in this species. Species-specific primer pairs were designed that differentiate E. histolytica from E. dispar as well as that reveal intraspecies PCR product length polymorphisms. The primers were tested with samples from different parts of...
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