Article
Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies.
Molecular and cellular probes - 1 Feb 2006
Ehricht Ralf, Slickers Peter, Goellner Stefanie, Hotzel Helmut, Sachse Konrad
Abstract excerpt
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This...
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