Article
HKT1 mediates sodium uniport in roots. Pitfalls in the expression of HKT1 in yeast.
Plant physiology - 1 Nov 2005
Haro Rosario, Bañuelos María A, Senn María E, Barrero-Gil Javier, Rodríguez-Navarro Alonso
Abstract excerpt
The function of HKT1 in roots is controversial. We tackled this controversy by studying Na+ uptake in barley (Hordeum vulgare) roots, cloning the HvHKT1 gene, and expressing the HvHKT1 cDNA in yeast (Saccharomyces cerevisiae) cells. High-affinity Na+ uptake was not detected in plants growing at high K+ but appeared soon after exposing the plants to a K(+)-free medium. It was a uniport, insensitive to external K+...
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