Article
[The discovery of a specific DNA fragment associated with maize cytoplasmic male sterility and its differential display].
Yi chuan = Hereditas - 1 Sept 2005
Cao Mo-Ju, Rong Ting-Zhao, Zhu Ying-Guo
Abstract excerpt
Three pairs of PCR primers were designed according to the mitochondrial DNA sequence. PCR amplification was applied to 3 sets of isonuclear alloplasm materials and 3 sets of isoplasm allonuclear materials. Multiplex PCR and general PCR protocol were adopted with total genomic DNA. As for the primers having detected polymorphsim between male sterility and its maintainers, differential display was conducted with...
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