Article
Purification and characterization of variants of acyl-CoA-binding protein in the bovine liver.
The Biochemical journal - 15 Jun 1992
Jensen M S, Højrup P, Rasmussen J T, Knudsen J
Abstract excerpt
Four differently modified forms of acyl-CoA-binding protein (ACBP) were identified in ACBP purified from bovine liver. The majority of the purified ACBP was focused at pH 5.9 in isoelectric focusing and could be shown to be N-acetylated ACBP without any further modifications. Two minor peaks were focused at pH 5.25 and 4.85 respectively. Mass spectrometry and sequence determination showed that the pI 5.25 form...
Topics
- Amino Acid Sequence
- Animals
- Carrier Proteins
- Cattle
- Diazepam Binding Inhibitor
- Fatty Acids
- Genetic Variation
- Isoelectric Focusing
- Liver
- Mass Spectrometry
- Molecular Sequence Data