Article
Improved diagnosis of mycobacterial infections in formalin‐fixed and paraffin‐embedded sections with nested polymerase chain reaction
1 Sept 2005
Abstract excerpt
Traditional histological diagnosis of mycobacterial infection in formalin-fixed and paraffin-embedded (FFPE) tissues is insensitive and poorly specific. To improve this, we developed nested polymerase chain reaction (PCR) protocols for detecting a Mycobacterium genus-specific 65-kDa heat shock protein (HSP65) sequence and the M. tuberculosis complex-specific insertion sequence IS6110 in FFPE sections. Protocols...
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