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Mammalian Leukocytes Contain All the Genetic Information Necessary for the Development of a New Individual

1 Sept 1999

Abstract excerpt

We have used leukocytes and oocytes from commercially slaughtered animals to clone a progeny tested Brown Swiss bull. Mononuclear cells were separated from the heparinized blood of the donor male on a Histopaque gradient and cryopreserved. The nuclei of thawed leukocytes were directly microinjected into enucleated Holstein Friesian oocytes that were subsequently activated. Development to morula was 23% and to...

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PMID: 16218815
DOI: 10.1089/15204559950019924

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