Article
Rupestris stem pitting associated virus isolates are composed by mixtures of genomic variants which share a highly conserved coat protein.
Archives of virology - 1 Jan 2006
Nolasco G, Santos C, Petrovic N, Teixeira Santos M, Cortez I, Fonseca F, Boben J, Nazaré Pereira A M, Sequeira O
Abstract excerpt
Broad spectrum primers were used to amplify a fragment comprising the CP gene and putative ORF6 by RT-PCR from ds-RNA templates originating from 46 Portuguese varieties, totalling 190 samples, including some wild Vitis ssp sylvestris vines, and 2 vines from Slovenia. SSCP analysis was used as a preliminary screen to avoid cloning and sequencing very similar variants. Four groups of variants were recognized. In...
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