Article
Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR.
Veterinary research - 1 Jan 2000
Geens Tom, Dewitte Angelo, Boon Nico, Vanrompay Daisy
Abstract excerpt
A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easily distinguish...
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