A multiplex PCR method for the detection of all five individual genes of <i>ica</i> locus in <i>Staphylococcus epidermidis</i>. A survey on 400 clinical isolates from prosthesis‐associated infections

Abstract excerpt

In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD,...

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