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Sugar beet BAC library construction and assembly of a contig spanning Rf1, a restorer-of-fertility gene for Owen cytoplasmic male sterility.

Molecular genetics and genomics : MGG - 1 Oct 2005

Hagihara Eiki, Matsuhira Hiroaki, Ueda Minoru, Mikami Tetsuo, Kubo Tomohiko

Abstract excerpt

Rf1 is a nuclear gene that controls fertility restoration in cases of cytoplasmic male sterility caused by the Owen cytoplasm in sugar beet. In order to isolate the gene by positional cloning, a BAC library was constructed from a restorer line, NK198, with the genotype Rf1Rf1. The library contained 32,180 clones with an average insert size of 97.8 kb, providing 3.4 genome equivalents. Five AFLP markers closely...

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PMID: 16080000
DOI: 10.1007/s00438-005-0024-5

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