Article
Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley.
Plant physiology - 1 Aug 2005
Hein Ingo, Barciszewska-Pacak Maria, Hrubikova Katarina, Williamson Sandie, Dinesen Malene, Soenderby Ida E, Sundar Suresh, Jarmolowski Artur, Shirasu Ken, Lacomme Christophe
Abstract excerpt
We successfully implemented virus-induced gene silencing (VIGS) in barley (Hordeum vulgare) for the functional characterization of genes required for Mla13-mediated resistance toward the biotrophic barley pathogen Blumeria graminis f. sp. hordei. Initially, barley cultivars were screened for their ability to host the barley stripe mosaic virus (BSMV)-VIGS vector by allowing its replication and systemic movement...
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