Article
Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin.
Journal of bacteriology - 1 Jun 1992
Delrue B, Fontaine T, Routier F, Decq A, Wieruszeski J M, Van Den Koornhuyse N, Maddelein M L, Fournet B, Ball S
Abstract excerpt
Amylose-defective mutants were selected after UV mutagenesis of Chlamydomonas reinhardtii cells. Two recessive nuclear alleles of the ST-2 gene led to the disappearance not only of amylose but also of a fraction of the amylopectin. Granule-bound starch synthase activities were markedly reduced in strains carrying either st-2-1 or st-2-2, as is the case for amylose-deficient (waxy) endosperm mutants of higher...
Topics
- Alleles
- Amylopectin
- Amylose
- Animals
- Chlamydomonas reinhardtii
- Chloroplasts
- Kinetics
- Mutation
- Selection, Genetic
- Starch
- Starch Synthase