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Loop-Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

1 Apr 2005

Abstract excerpt

We have evaluated a diagnostic system based on the loop-mediated isothermal amplification (LAMP) assay for the rapid, simple, and sensitive detection of Newcastle disease virus (NDV) directly from culture isolates as well as clinical samples. By using one set of specific primers targeting the fusion protein gene, the LAMP assay rapidly amplified the target gene within 2 h, requiring only a regular laboratory...

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PMID: 15814979
DOI: 10.1128/jcm.43.4.1646-1650.2005

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