Article
Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.
Applied and environmental microbiology - 1 Apr 2005
Tamaki Hideyuki, Sekiguchi Yuji, Hanada Satoshi, Nakamura Kazunori, Nomura Nakao, Matsumura Masatoshi, Kamagata Yoichi
Abstract excerpt
Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the...
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