Article
Mutations in subunits of the Escherichia coli twin-arginine translocase block function via differing effects on translocation activity or tat complex structure.
Journal of molecular biology - 25 Mar 2005
Barrett Claire M L, Mangels Dorothea, Robinson Colin
Abstract excerpt
We have used a combination of blue-native (BN) gel electrophoresis and protein purification to analyze the effects of TatA or TatC mutations on the structures of the primary TatABC and multimeric TatA complexes in Escherichia coli. Expression of wild-type TatABC leads to the production of a singl...
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