Article
Mass spectrometric analysis of the HIV-1 integrase-pyridoxal 5'-phosphate complex reveals a new binding site for a nucleotide inhibitor.
The Journal of biological chemistry - 4 Mar 2005
Williams Kerry L, Zhang Yijun, Shkriabai Nick, Karki Rajeshri G, Nicklaus Marc C, Kotrikadze Nana, Hess Sonja, Le Grice Stuart F J, Craigie Robert, Pathak Vinay K, Kvaratskhelia Mamuka
Abstract excerpt
HIV-1 integrase (IN) is an important target for designing new antiviral therapies. Screening of potential inhibitors using recombinant IN-based assays has revealed a number of promising leads including nucleotide analogs such as pyridoxal 5'-phosphate (PLP). Certain PLP derivatives were shown to also exhibit antiviral activities in cell-based assays. To identify an inhibitory binding site of PLP to IN, we used...
Topics
- Amino Acid Sequence
- Binding Sites
- Cell Line, Tumor
- Cross-Linking Reagents
- DNA
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- HIV Integrase
- Humans
- Inhibitory Concentration 50