Article
Genetics and geographical variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand.
Veterinary microbiology - 10 Jun 2004
Thanawongnuwech R, Amonsin A, Tatsanakit A, Damrongwatanapokin S
Abstract excerpt
The Thai isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were obtained from the Chulalongkorn University-Veterinary Diagnostic Laboratory (CU-VDL). Virus isolation was confirmed by immunoperoxidase monolayer assay (IPMA) using SDOW-17. The virus genotype was determined using nested multiplex RT-PCR (nm RT-PCR) of ORF 1b. The nm RT-PCR was able to detect at least 10TCID50/ml of PRRSV. Of...
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