Article
Characterization of heterotrimeric G protein complexes in rice plasma membrane.
The Plant journal : for cell and molecular biology - 1 Apr 2004
Kato Chiyuki, Mizutani Tomohiro, Tamaki Hisanori, Kumagai Hidehiko, Kamiya Takehiro, Hirobe Ayumi, Fujisawa Yukiko, Kato Hisaharu, Iwasaki Yukimoto
Abstract excerpt
Two genes in the rice genome were identified as those encoding the gamma subunits, gamma1 and gamma2, of heterotrimeric G proteins. Using antibodies against the recombinant proteins for the alpha, beta, gamma1, and gamma2 subunits of the G protein complexes, all of the subunits were proven to be localized in the plasma membrane in rice. Gel filtration of solubilized plasma membrane proteins showed that all of the...
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