Article
[Genomic DNA fingerprinting by pulsed-field gel electrophoresis for study of nosocomial infection caused by methicillin-resistant Staphylococcus aureus].
Nihon rinsho. Japanese journal of clinical medicine - 1 May 1992
Ichiyama S
Abstract excerpt
In this study, we have compared genomic DNA fingerprintings among isolates of methicillin-resistant Staphylococcus aureus (MRSA) by using pulsed-field gel electrophoresis (PFGE). Chromosomal fragments digested with Sma I were most suitable for the PFGE separation, giving 15-20 fragments of sizes ranging from about 30-1,500 kb. Thirty-one distinctive fragment patterns were identified in 111 isolates of MRSA. On...
Topics
- Cross Infection
- DNA Fingerprinting
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Methicillin Resistance
- Staphylococcal Infections
- Staphylococcus aureus
