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Article

Nipah virus conforms to the rule of six in a minigenome replication assay

1 Mar 2004

Abstract excerpt

To study the replication of Nipah virus (NiV), a minigenome replication assay that does not require the use of infectious virus was developed. The minigenome was constructed to encode a NiV vRNA analogue containing the gene for chloramphenicol acetyltransferase (CAT) under the control of putative NiV transcription motifs and flanked by the NiV genomic termini. CAT protein was detected only when plasmids encoding...

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PMID: 14993656
DOI: 10.1099/vir.0.19685-0

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