Article
Phylogenetic diversity of dissimilatory sulfite reductase genes from deep-sea cold seep sediment.
Marine biotechnology (New York, N.Y.) - 1 Jan 2000
Fukuba Tatsuhiro, Ogawa Mari, Fujii Teruo, Naganuma Takeshi
Abstract excerpt
The phylogenetic diversity of dissimilatory sulfite reductase (DSR, EC 1.8.99.3) alpha-subunit genes from a deep-sea cold seep was analyzed. Bulk genomic DNA was extracted from the cold seep sediment and used for amplification by polymerase chain reaction (PCR) of DSR alpha-subunit gene. Two sizes of PCR products, 1.4 kb (expected) and 1.3 kb (unexpected), were amplified. Sixteen clones of the 1.4-kb amplicons...
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