Article
Novel mass spectrometry-based tool for genotypic identification of mycobacteria.
Journal of clinical microbiology - 1 Jan 2004
Lefmann Michael, Honisch Christiane, Böcker Sebastian, Storm Niels, von Wintzingerode Friedrich, Schlötelburg Cord, Moter Annette, van den Boom Dirk, Göbel Ulf B
Abstract excerpt
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR amplified and in vitro-transcribed 16S rRNA gene (rDNA) was used for the identification of mycobacteria. Full-length 16S rDNA reference sequences of 12 type strains of Mycobacterium spp. frequently isolated from clinical specimens were determined by PCR, cloning, and sequencing. For...
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