Article
Nucleotide variation in the VP7 gene affects PCR genotyping of G9 rotaviruses identified in Italy.
Journal of medical virology - 1 Jan 2004
Martella Vito, Terio Valentina, Arista Serenella, Elia Gabriella, Corrente Marialaura, Madio Anna, Pratelli Annamaria, Tempesta Maria, Cirani Antonio, Buonavoglia Canio
Abstract excerpt
A modified (aFT9m) and a degenerate (aFT9d) version of the rotavirus G9-specific primer (aFT9) allowed strains that were previously untypable, because of point mutations accumulating at the primer binding site, to be G typed by reverse transcription-polymerase chain reaction. The strains were collected during 2001-2002 in Italy in hospitals of the Apulia region, from children affected by severe...
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