Article
Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique.
Journal of dentistry - 1 Nov 2003
Tang Gaoyan, Samaranayake Lakshman P, Yip Hak-Kong, Chu Frederick C S, Tsang Peter C S, Cheung Becky P K
Abstract excerpt
OBJECTIVES: The poor sensitivity of phenotypic identification techniques has hampered the taxonomic differentiation of Actinomyces. Hence we developed a sensitive and specific, PCR-based oligonucleotide-DNA hybridization technique to detect Actinomyces spp. and, used this method to detect these organisms in samples directly obtained from infected root canals. METHODS: A total of 32 samples from 28 Chinese...
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