Article
Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction.
Journal of clinical microbiology - 1 Oct 1992
van Belkum A, De Jonckheere J, Quint W G
Abstract excerpt
All six Naegleria species recognized to date were studied by interrepeat polymerase chain reaction (PCR). Priming at repeat sequences, which are known to be variable among eukaryotes, yielded electrophoretic DNA banding patterns that were specific for any single species. With a single PCR and simple gel electrophoresis, species determination could be performed in less than 1 day. Unambiguous discrimination...
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