Article
Differentiation of isolates of Discula umbrinella (Teleomorph: Apiognomonia errabunda) from beech, chestnut, and oak using randomly amplified polymorphic DNA markers.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Haemmerli U A, Brändle U E, Petrini O, McDermott J M
Abstract excerpt
Genetic variation in 30 isolates of Discula umbrinella derived from beech, chestnut, and oak was assessed using randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphic markers. Polymerase chain reaction amplifications with 17 primers produced 134 different DNA fragments. Three RAPD fragments were subsequently used for Southern hybridization. By these techniques up to four different...
Topics
- Base Sequence
- DNA, Single-Stranded
- Genetic Markers
- Genetic Variation
- Mitosporic Fungi
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Trees