Article
Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.
Journal of bacteriology - 1 Dec 1992
Kung S S, Chen J, Chow W Y
Abstract excerpt
An insertion element, ISAE1, was discovered during the molecular analysis of mutants defective in the autotrophic growth (Aut-) of Alcaligenes eutrophus H1-4, a mitomycin C-generated derivative of strain H1. ISAE1 is 1,313 bp long, has 12-bp nearly perfect inverted terminal repeats, and contains an open reading frame that has a coding capacity of 408 amino acids. Direct repeats of 8 bp were generated by insertion...
Topics
- Alcaligenes
- Bacterial Proteins
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Molecular Sequence Data
- Mutation
- Phenotype
- Restriction Mapping
