Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1.

doi:10.1016/0042-6822(92)90262-n

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Article

Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1.

Virology - 1 Dec 1992

Deng R, Brock K V

Abstract excerpt

Genomic RNA of noncytopathic (NCP) bovine viral diarrhea virus (BVDV) strain SD-1 was extracted directly from serum obtained from a persistently infected animal. cDNA was synthesized and amplified by polymerase chain reaction (PCR) before cloning. The complete genomic nucleotide sequence was determined by sequencing at least two different clones from independent PCR reactions. The 5' and 3' end sequences of the...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
Cytopathogenic Effect, Viral
Genetic Variation
Genome, Viral
Molecular Sequence Data
Open Reading Frames
Pestivirus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Protein Precursors

Protein Structure, Secondary

RNA, Viral

Sequence Homology, Amino Acid

Sequence Homology, Nucleic Acid

Viral Proteins

Source

Open in PubMed

Identifiers

PMID: 1333126
DOI: 10.1016/0042-6822(92)90262-n

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1333126-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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