Article
A different molecular interaction of bradykinin and the synthetic agonist FR190997 with the human B2 receptor: evidence from mutational analysis.
British journal of pharmacology - 1 Oct 2003
Bellucci Francesca, Meini Stefania, Cucchi Paola, Catalani Claudio, Reichert Wolfgang, Zappitelli Sabrina, Rotondaro Luigi, Quartara Laura, Giolitti Alessandro, Maggi Carlo Alberto
Abstract excerpt
Binding affinity at the [3H]-BK binding site and activity as inositol phosphate (IP) production by the peptide bradykinin (BK) and the nonpeptide FR190997 were studied at wild-type or point-mutated human B2 receptors (hB2R) expressed in CHO cells. The effect of the following mutations were analyzed: E47A (TM1), W86A and T89A (TM2), I110A, L114A and S117A (TM3), T158A, M165T and L166F (TM4), T197A and S211A (TM5),...
Topics
- Animals
- Bradykinin
- CHO Cells
- Cricetinae
- DNA Mutational Analysis
- Dose-Response Relationship, Drug
- Humans
- Mutation
- Protein Binding
- Quinolines
- Receptor, Bradykinin B2