Use of Real-Time PCR with Multiple Targets To Identify <i>Pseudomonas aeruginosa</i> and Other Nonfermenting Gram-Negative Bacilli from Patients with Cystic Fibrosis

Abstract excerpt

Pseudomonas aeruginosa and other gram-negative isolates from patients with cystic fibrosis (CF) may be difficult to identify because of their marked phenotypic diversity. We examined 200 gram-negative clinical isolates from CF respiratory tract specimens and compared identification by biochemical testing and real-time PCR with multiple different target sequences using a standardized combination of biochemical...

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