Article
Molecular characterization of microsporidia indicates that wild mammals Harbor host-adapted Enterocytozoon spp. as well as human-pathogenic Enterocytozoon bieneusi.
Applied and environmental microbiology - 1 Aug 2003
Sulaiman Irshad M, Fayer Ronald, Lal Altaf A, Trout James M, Schaefer Frank W, Xiao Lihua
Abstract excerpt
Over 13 months, 465 beavers, foxes, muskrats, otters, and raccoons were trapped in four counties in eastern Maryland and examined by molecular methods for microsporidia. A two-step nested PCR protocol was developed to amplify a 392-bp fragment of the internal transcribed spacer region of the rRNA gene of Enterocytozoon spp., with the use of primers complementary to the conserved regions of published nucleotide...
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