Article
An improved amplified fragment length polymorphism (AFLP) protocol for discrimination of Listeria isolates.
Systematic and applied microbiology - 1 Jun 2003
Keto-Timonen Riikka O, Autio Tiina J, Korkeala Hannu J
Abstract excerpt
Nine restriction enzyme combinations and 108 different primer combinations were initially tested for suitability for amplified fragment length polymorphism (AFLP) analysis of Listeria monocytogenes; the combination of HindIII and HpyCH4IV showed consistently strong signals on gels, amplified an adequate number of DNA fragments and detected polymorphism among closely related strains based on AscI macrorestriction...
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