Article
Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification.
Experimental parasitology - 1 Jan 2000
Yang Zhao-Qing, Li Qing-Qing, Zuo Yang-Xian, Chen Xin-Wen, Chen Yong-Jiu, Nie Long, Wei Chang-Gue, Zen Jia-Shun, Attwood S W, Zhang Xue-Zheng, Zhang Ya-Ping
Abstract excerpt
Thirteen restriction endonucleases were used to investigate nuclotide sequence variation in the 18S rRNA DNA of 88 individuals from ten Sarcocystis taxa collected as cysts from their intermediate hosts, swine, cattle and water buffalo. A DNA sequence of approximately 900 bp was used. A total of 26 electromorphs were detected. The electromorphs were sorted into seven different haplotypes that coincided with the...
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