Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures.

doi:10.1128/JCM.41.7.3336-3338.2003

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Article

Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures.

Journal of clinical microbiology - 1 Jul 2003

Park Chang-Young, Kwak Minjung, Gutierrez Oscar, Graham David Y, Yamaoka Yoshio

Abstract excerpt

PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.

Topics

Antigens, Bacterial
Bacterial Proteins
Bacterial Typing Techniques
Biopsy
Culture Media
DNA, Bacterial
Genotype
Helicobacter Infections
Helicobacter pylori
Humans
Polymerase Chain Reaction
Stomach

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Identifiers

PMID: 12843087
DOI: 10.1128/JCM.41.7.3336-3338.2003
PMCID: PMC165374

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