Article
Direct identification and enrichment of retinal stem cells/progenitors by Hoechst dye efflux assay.
Investigative ophthalmology & visual science - 1 Jun 2003
Bhattacharya Sumitra, Jackson John D, Das Ani V, Thoreson Wallace B, Kuszynski Charles, James Jackson, Joshi Shantaram, Ahmad Iqbal
Abstract excerpt
PURPOSE: The present study describes a method for isolating neural stem cells/progenitors directly from the freshly dissociated embryonic retina (prospective identification) and compares their characteristics with those enriched from mitogen-exposed embryonic retinal cell culture. METHODS: Cell dissociates from embryonic rat retina and mitogen-exposed embryonic retinal cultures were stained with Hoechst 33342...
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