Article
Evaluation of different primers for detecting mecA gene by PCR in comparison with phenotypic methods for discrimination of methicillin-resistant Staphylococcus aureus.
The Southeast Asian journal of tropical medicine and public health - 1 Dec 2002
Siripornmongcolchai Taweeporn, Chomvarin Chariya, Chaicumpar Kunyaluk, Limpaiboon Temduang, Wongkhum Chaisiri
Abstract excerpt
Detection of the mecA gene by polymerase chain reaction (PCR) is the gold standard for identifying methicillin-resistant Staphylococcus aureus (MRSA). PCR assays, employing MR1-MR2 primers (primer set 1) and MR3-MR4 primers (primer set 2) to generate 154 and 533 bp fragment, respectively, are most widely used for amplification of mecA gene. The purpose of this study was to evaluate the presence of mecA gene in...
Topics
- Bacterial Proteins
- Carrier Proteins
- Colony Count, Microbial
- DNA Primers
- DNA, Bacterial
- Diagnosis, Differential
- Electrophoresis, Agar Gel
- Hexosyltransferases
- Histocompatibility Antigens Class I
- Humans
- Methicillin Resistance
- Microbial Sensitivity Tests