Article
Genotypic discrimination between a Leishmania isolate and two Leishmania major reference strains using PCR-RFLP technique.
Journal of the Egyptian Society of Parasitology - 1 Apr 2003
el-Kerdany Eman D, Abd-el-Haleem Desouky
Abstract excerpt
The restriction fragment length polymorphism (PCR-RFLP) of 18S rDNA amplified fragments, was conducted to recognize between an unidentified Leishmania isolated from Egyptian patient infected in Saudi Arabia and two L. major reference strains causing cutaneous lesions. The strains were maintained both in vivo & in vitro. Additional requirement as morphological characterization on basis of the light microscope &...
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