Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring.

doi:10.1007/BF02828173

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Article

Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban - 1 Jan 2002

Guo Jianli, Sun Yaliang

Abstract excerpt

The luminous intensity of dark variant (S1) separated from photobacterium phosphoreum (A2) was 1/10,000 less than that of wild-type. Ethidium bromide (EB) (0.6 mg/L), Mytomycin C (MC, 0.05 mg/L), 2-amino fluorene (2-AF, 1.0 mg/L) all could strongly induce reversion mutation for S1 within 24 h and increase reversion ratio significantly. The results of experiments indicated that these revertants had stable genetic...

Topics

Ethidium
Genetic Variation
Luciferases
Luminescent Measurements
Mitomycins
Mutagens
Mutation
Photobacterium
Toxicology
Transcription, Genetic

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Open in PubMed

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PMID: 12658797
DOI: 10.1007/BF02828173

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