Article
The role of copper and protons in heme-copper oxidases: kinetic study of an engineered heme-copper center in myoglobin.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 2003
Sigman Jeffrey A, Kim Hyeon K, Zhao Xuan, Carey James R, Lu Yi
Abstract excerpt
To probe the role of copper and protons in heme-copper oxidase (HCO), we have performed kinetic studies on an engineered heme-copper center in sperm whale myoglobin (Leu-29 --> HisPhe-43 --> His, called Cu(B)Mb) that closely mimics the heme-copper center in HCO. In the absence of metal ions, the engineered Cu(B) center in Cu(B)Mb decreases the O(2) binding affinity of the heme. However, addition of Ag(I), a...
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