Article
Evaluation of Semiautomated Multiplex PCR Assay for Determination of <i>Streptococcus pneumoniae</i> Serotypes and Serogroups
1 Feb 2003
Abstract excerpt
A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresis with a 7-min run time, and a serotype or serogroup was assigned on the basis of fragment size. The method was used...
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