Article
The yeast counterparts of human 'MELAS' mutations cause mitochondrial dysfunction that can be rescued by overexpression of the mitochondrial translation factor EF-Tu.
EMBO reports - 1 Jan 2003
Feuermann M, Francisci S, Rinaldi T, De Luca C, Rohou H, Frontali L, Bolotin-Fukuhara M
Abstract excerpt
We have taken advantage of the similarity between human and yeast (Saccharomyces cerevisiae) mitochondrial tRNA(Leu)(UUR), and of the possibility of transforming yeast mitochondria, to construct yeast mitochondrial mutations in the gene encoding tRNA(Leu)(UUR) equivalent to the human A3243G, C3256T and T3291C mutations that have been found in patients with the neurodegenerative disease MELAS (for mitochondrial...
Topics
- Amino Acid Substitution
- Base Sequence
- Biolistics
- DNA, Mitochondrial
- Gene Expression Regulation, Fungal
- Genetic Vectors
- Humans
- MELAS Syndrome
- Mitochondria
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation, Missense
- Peptide Elongation Factor Tu