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Article

Ordered cosmid library of the Mesorhizobium loti MAFF303099 genome for systematic gene disruption and complementation analysis.

Plant & cell physiology - 1 Dec 2002

Hattori Yoshiyuki, Omori Hirofumi, Hanyu Masaki, Kaseda Noriko, Mishima Elina, Kaneko Takakazu, Tabata Satoshi, Saeki Kazuhiko

Abstract excerpt

For effective exploitation of the genome sequence information of Lotus microsymbiont, Mesorhizobium loti MAFF303099, to discover gene functions, we have constructed an ordered and mutually overlapping cosmid library using an IncP broad host-range vector. The library consisted of 480 clones to cover approximately 99.6% of the genome with average insert size and overlap of 26.9 and 11.1 kbp, respectively. The...

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PMID: 12514252
DOI: 10.1093/pcp/pcf175

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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