Article
Identification and evaluation of major histocompatibility complex antigens in chicken chimeras and their relationship to germline transmission.
Poultry science - 1 Oct 2002
Bacon L D, Zajchowski L, Clark M E, Etches R J
Abstract excerpt
Chimeric chickens were evaluated as an intermediate for development of transgenic chickens. The transfer of Barred Plymouth Rock (BR) blastodermal cells into White Leghorn (WL) embryos results in BR-->WL chimeras, and some breeder males generate over 30% germline transmission of the BR genotype to offspring based on a feather-color trait. The objectives of the current study were to 1) identify the MHC (B...
Topics
- Animals
- Animals, Genetically Modified
- Blastoderm
- Breeding
- Chick Embryo
- Chickens
- Chimera
- Erythrocytes
- Female
- Gene Expression
- Genotype
- Haplotypes
- Heredity
- Histocompatibility Antigens
- Major Histocompatibility Complex
- Male
- Polymerase Chain Reaction
- Transfection