Article
[Application of PCR technique in genetic diagnosis of Duchenne/Becker muscular dystrophy].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Aug 2002
Liu Yong-Mei, Feng Zhi-Chun, Fang Zhen-Wei
Abstract excerpt
OBJECTIVE: To study the application of PCR technique in genetic detection of Duchenne/Becker muscular dystrophy (DMD/BMD). METHODS: A multiple PCR system is established according to the multiple sites of DMD/BMD exon deletion. Under different PCR conditions, multiple exon deletions, single-strand conformation polymorphism, allopolyploid, chain labeling, restriction fragment length polymorphism and microsatellite...
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