Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species.

doi:10.1128/IAI.70.10.5670-5675.2002

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Article

Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species.

Infection and immunity - 1 Oct 2002

Akiyoshi D E, Feng X, Buckholt M A, Widmer G, Tzipori S

Abstract excerpt

Cryptosporidium parvum TU502, a genotype 1 isolate of human origin, was passaged through three different mammalian hosts, including humans, pigs, and calves. It was confirmed to be genotype 1 by PCR-restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein gene, direct sequencing of PCR fragments of the small subunit rRNA and beta-tubulin genes, and microsatellite analysis. This...

Topics

Animals
Base Sequence
Cattle
Cryptosporidium parvum
DNA, Bacterial
Genes, Bacterial
Genotype
Germ-Free Life
Humans
Mice
Mice, Knockout
Microsatellite Repeats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Protozoan Proteins

RNA, Bacterial

RNA, Ribosomal

Source

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Identifiers

PMID: 12228296
DOI: 10.1128/IAI.70.10.5670-5675.2002
PMCID: PMC128364

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